|
Status |
Public on Dec 01, 2017 |
Title |
U937 cells transduced with pCDH-MSCV-puro-DNMT3A-WT lentivirus vector_methylation |
Sample type |
genomic |
|
|
Source name |
Human promyelocytic leukemia cell line_U937_DNMT3A_wildtype
|
Organism |
Homo sapiens |
Characteristics |
cell line: U937 cell type: Human promyelocytic leukemia cell line tissue: Blood
|
Treatment protocol |
Cultured U937 cells were infected with lentivirus in the presence of 8 µg/ml polybrene (Sigma) and cultured for 72 hours. The infected cells were selected with 2 µg/mL puromycin for 2-weeks to obtain stable clones. DNMT3A WT/mutants expression was checked by immunoblot analysis and stable cells used for DNA extraction for methylation microarray analysis.
|
Growth protocol |
The human promyelocytic leukemia cells, U937 were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 1× antibiotic-antimycotic (Gibco by Life Technologies) in a humidified chamber with 5% CO2 atmosphere at 37°C
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Total DNA was isolated by standard procedures
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina labelling protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina MethylationEPIC BeadChip microarray using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
Data processing |
Genome Studio V2011.1
|
|
|
Submission date |
Dec 06, 2016 |
Last update date |
Dec 01, 2017 |
Contact name |
Yun-Shien Lee |
E-mail(s) |
bojack@mail.mcu.edu.tw
|
Organization name |
Ming Chuan Univ
|
Department |
Dept. Biotechnology
|
Lab |
GMRCL
|
Street address |
TauYau
|
City |
TauYau |
State/province |
Taiwan |
ZIP/Postal code |
115 |
Country |
Taiwan |
|
|
Platform ID |
GPL21145 |
Series (2) |
GSE90933 |
Differential genomic-methylation profile of U937 cells transduced with WT/mutant-DNMT3A |
GSE90934 |
Differential gene expression and genomic methylation profile of U937 cells transduced with WT/mutant-DNMT3A |
|