|
| Status |
Public on Mar 09, 2017 |
| Title |
Stab2Cre;Gata4 heterozygous biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
control fetal liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: fetal liver
genotype/variation: Stab2Cre;Gata4 heterozygous
|
| Treatment protocol |
Timed matings were performed, the embryos were harvested at embryonic day 11.5 and the fetal liver was dissected.
|
| Growth protocol |
Stab2-Cre mice were crossed to mouse lines bearing floxed alleles of Gata4 (B6;129-Gata4tm1.1Sad/J; Jax#008194]. To obtain loss-of-function and control genotypes Stab2-Cretg/wt;Gata4fl/wt were crossed to Gata4fl/fl mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total was RNA from Stab2-Cre;Gata4fl/fl or control fetal liver was extracted with High Pure RNA isolation Kit according to the manufacturer's protocol (Roche).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from control fetal liver
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 18 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Dec 13, 2016 |
| Last update date |
Mar 09, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18802 |
| Series (2) |
| GSE92352 |
GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis (1 of 3) |
| GSE92357 |
GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis |
|
