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Sample GSM242969 Query DataSets for GSM242969
Status Public on Dec 09, 2008
Title Ferret lung with H3N2 at Day 2, biological rep2
Sample type RNA
 
Source name Ferret
Organism Mustela putorius furo
Characteristics Tissue: lung
Virus Strain: H3N2
Treatment protocol Ferrets were inoculated intranasally with 10(6) EID50 of either A/Vietnam/1203/04 (H5N1) or A/Panama/2007/99 (H3N2). At 2, 4 and 6 days post-infection (DPI), ferrets were euthanized and lung tissue was excised and placed on ice in the TRIzol solution (Invitrogen) and homogenized immediately.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from ferret on Day 2 after infection
Data processing The data were analyzed with PLIER using ArrayAssist (Stratagene) default analysis settings.
 
Submission date Nov 14, 2007
Last update date Aug 14, 2011
Contact name Longsi Ran
E-mail(s) lran@uhnresearch.ca
Phone 416-581-7607
Organization name Toronto General Research Institute
Department Division of Experimental Therapeutics
Lab Dr. David J Kelvin
Street address 101 College Street
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL3738
Series (1)
GSE9606 Lungs of ferrets infected with either an H3N2 or H5N1 subtype of influenza A virus.

Data table header descriptions
ID_REF
VALUE PLIER-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 838.11597
AFFX-BioB-M_at 1100.5945
AFFX-BioB-3_at 804.21326
AFFX-BioC-5_at 1876.49
AFFX-BioC-3_at 1719.6646
AFFX-BioDn-5_at 3123.892
AFFX-BioDn-3_at 3234.7256
AFFX-CreX-5_at 8139.8154
AFFX-CreX-3_at 7451.7373
AFFX-DapX-5_at 206.62299
AFFX-DapX-M_at 2307.6704
AFFX-DapX-3_at 4205.491
AFFX-LysX-5_at 38.988052
AFFX-LysX-M_at 449.15207
AFFX-LysX-3_at 1764.9418
AFFX-PheX-5_at 147.61389
AFFX-PheX-M_at 802.46735
AFFX-PheX-3_at 1296.3824
AFFX-ThrX-5_at 115.90695
AFFX-ThrX-M_at 611.4595

Total number of rows: 43035

Table truncated, full table size 1262 Kbytes.




Supplementary file Size Download File type/resource
GSM242969.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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