|
| Status |
Public on Nov 27, 2007 |
| Title |
Rat Hair cells from crista ampullaris_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat crista ampullaris
|
| Organism |
Rattus norvegicus |
| Characteristics |
Brown norwary rats, 6-8 weeks old, crista ampullaris
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Arcturus Picopur RNA Isolation Kit (Arcturus Bioscience, Mountain View, CA)
|
| Label |
Cy5
|
| Label protocol |
Biotin-labeled cRNA was prepared by linear amplification of the Poly(A)+ RNA population within the total RNA sample
|
| |
|
| Hybridization protocol |
Arrays were hybridized at 37oC for 20h in a shaking incubator.
|
| Scan protocol |
Dried arrays were scanned with a GenePixTM 4000B scanner (Molecular Devices, Axon Instruments, Union City, CA).
|
| Description |
Rat crista ampullaris, laser captured based on morphology
|
| Data processing |
Raw values represent the final number of the spot after the background is subtracted out. Normalized values represent median global normalization method, i.e. raw intensity/median intensity of all spots on the array
|
| |
|
| Submission date |
Nov 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Ashley Wackym |
| Organization name |
Medical College of Wisconsin
|
| Department |
Otolaryngology
|
| Lab |
Otolaryngology Research
|
| Street address |
9200 W. Wisconsin Ave
|
| City |
Milwaukee |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platform ID |
GPL2896 |
| Series (1) |
| GSE9684 |
Trophic Factor Gene Expression in Supporting Cells of the Crista Ampullaris:Implications for Hair Cell Regeneration |
|
