|
| Status |
Public on Jan 11, 2017 |
| Title |
LY_B cells_Female_rep1 |
| Sample type |
genomic |
| |
|
| Source name |
B cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
|
| Treatment protocol |
We cultured the cell lines for 4-6 weeks until the cultures contained 20 million cells.
|
| Growth protocol |
Mononuclear cells were isolated by density gradient centrifugation in Ficoll-Hypaque. B-cells were isolated by positive selection using CD19 Dynabeads PanB magnetic beads (Invitrogen).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from B cells and LCLs samples using Qiagen DNeasy Kit according to standard instructions
|
| Label |
cy3，cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
normal B cells sample
LY-Blood-2.AVG_Beta
|
| Data processing |
GenomeStudio and Minfi R package
|
| |
|
| Submission date |
Jan 10, 2017 |
| Last update date |
Jan 11, 2017 |
| Contact name |
Rui Zhang |
| E-mail(s) |
zrus116@163.com
|
| Phone |
13051152071
|
| Organization name |
Peking University Cancer Hospital & Institute
|
| Street address |
52 Fucheng Road
|
| City |
Beijing |
| ZIP/Postal code |
100142 |
| Country |
China |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE93373 |
Epigenome analysis of B cell and B cells infected with Epstein-Barr virus (EBV) |
|
