|
| Status |
Public on Dec 04, 2007 |
| Title |
Reversine treated C2C12 Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse myoblast
|
| Organism |
Mus musculus |
| Characteristics |
reversine-treated C2C12 cells
|
| Treatment protocol |
C2C12 cells were treated with DMSO (control) or 5uM reversine.
|
| Growth protocol |
The C2C12 cell line, which is mouse myoblast, was cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using easy-Blue reagent (iNtRON Biotechnology, Sungnam, Gyeonggi, Korea). RNA was stored at -70C in Rnase free H20 supplemented with the Rnase inhibitor. Quality and quantity of RNA was determined using a Agilent Bioanalyzer as per manufacturer procedures(Agilent Technologies, Palo Alto, CA)
|
| Label |
DIG-UTP
|
| Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT kit V2.0.
|
| |
|
| Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imageing, auto gridding and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1. 0. 3.
|
| Description |
C2C12 cells were treated with 5mM reversine. for 4 days.Microarrays were performed in duplicated, R1 and R2
|
| Data processing |
Signal intensities across microarrays were normalized using the quantile-quantile method (www.bioconductor.org).
|
| |
|
| Submission date |
Dec 03, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Eun Kyung Lee |
| Organization name |
Sungkyunkwan university
|
| Department |
pharmacy
|
| Lab |
molecular biology
|
| Street address |
300 Cheoncheon-dong, Jangan-gu
|
| City |
suwon |
| State/province |
Gyeonggi-do |
| ZIP/Postal code |
440-746 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE9754 |
Murine myoblasts : Control vs. Reversine treated cells |
|
