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Sample GSM2467280 Query DataSets for GSM2467280
Status Public on Apr 23, 2018
Title LTAD Ethanol treated siCOBLL1
Sample type SRA
 
Source name prostate cancer cells
Organism Homo sapiens
Characteristics cell line: LTAD cells
treatment: ethanol 24h siCOBLL1 treated
Treatment protocol Cells were transfected with siRNA for 48 h and treated with vehicle or DHT 10 nM for 24 h.
Growth protocol LNCaP cells were cultures in RPMI 1640 containing 10% FBS. LTAD cells were treated with phenol-red free RPMI1640 containing 10% charcoal/dextran treated FBS. Before hormone treatment, cells were incubated in phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using ISOGEN reagent.
RNA sequencing (RNA-seq) library construction and sequencing was performed according to the manufacturer’s protocol for the Applied Biosystems SOLiD 4 System (Applied Biosystems).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model AB SOLiD 4 System
 
Data processing The SOLiD-generated RNA-Seq reads was in 50 bp length and an initial filtering process was taken to remove any non-desirable contamination sequences.
The reads were mapped to the human RefSeq mRNA database.
The expression levels of mapped transcripts were normalized into Reads Per Kilobase of exon per Million mapped reads (RPKM) to faciliate comparison among different samples.
Genome_build: hg19
Supplementary_files_format_and_content: Excel file of RPKM values for each Sample
 
Submission date Jan 24, 2017
Last update date May 15, 2019
Contact name Ken-ichi Takayama
Organization name Tokyo Metropolitan Institute of Gerontology
Street address Sakaecho
City Itabashi-ku
ZIP/Postal code 173-0015
Country Japan
 
Platform ID GPL13393
Series (1)
GSE94028 The role of AR associated protein and lncRNA in androgen signaling
Relations
BioSample SAMN06255918
SRA SRX2513958

Supplementary file Size Download File type/resource
GSM2467280_refseq.hg19.ltad_Et_siCOBLL1.xlsx 5.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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