|
Status |
Public on Apr 23, 2018 |
Title |
LTAD Ethanol treated siCOBLL1 |
Sample type |
SRA |
|
|
Source name |
prostate cancer cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: LTAD cells treatment: ethanol 24h siCOBLL1 treated
|
Treatment protocol |
Cells were transfected with siRNA for 48 h and treated with vehicle or DHT 10 nM for 24 h.
|
Growth protocol |
LNCaP cells were cultures in RPMI 1640 containing 10% FBS. LTAD cells were treated with phenol-red free RPMI1640 containing 10% charcoal/dextran treated FBS. Before hormone treatment, cells were incubated in phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using ISOGEN reagent. RNA sequencing (RNA-seq) library construction and sequencing was performed according to the manufacturer’s protocol for the Applied Biosystems SOLiD 4 System (Applied Biosystems).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
AB SOLiD 4 System |
|
|
Data processing |
The SOLiD-generated RNA-Seq reads was in 50 bp length and an initial filtering process was taken to remove any non-desirable contamination sequences. The reads were mapped to the human RefSeq mRNA database. The expression levels of mapped transcripts were normalized into Reads Per Kilobase of exon per Million mapped reads (RPKM) to faciliate comparison among different samples. Genome_build: hg19 Supplementary_files_format_and_content: Excel file of RPKM values for each Sample
|
|
|
Submission date |
Jan 24, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Ken-ichi Takayama |
Organization name |
Tokyo Metropolitan Institute of Gerontology
|
Street address |
Sakaecho
|
City |
Itabashi-ku |
ZIP/Postal code |
173-0015 |
Country |
Japan |
|
|
Platform ID |
GPL13393 |
Series (1) |
GSE94028 |
The role of AR associated protein and lncRNA in androgen signaling |
|
Relations |
BioSample |
SAMN06255918 |
SRA |
SRX2513958 |