|
| Status |
Public on Dec 01, 2008 |
| Title |
Contralateral LGN layer 6 (deprived layer) of Monocular Deprived for 2 months |
| Sample type |
RNA |
| |
|
| Source name |
Lateral Geniculate Nucleus underwent Monocular Deprived for 2 months
|
| Organism |
Macaca mulatta |
| Characteristics |
Lateral Geniculate Nucleus were used from male Macaque monkeys using opaque dark contact lens underwent Monocular Deprived for 2 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Laser Capture Microdissection was used to cut each individual layer from each Lateral Geniculate Nucleus. Total RNA from each layer was isolated using the PicoPure RNA kit from Arcturus.
|
| Label |
biotinylated cRNA
|
| Label protocol |
Two rounds of linear T7-based RNA amplification was used utilizing T7 RNA polymerase.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol was used in an Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
Hewlett-Packard Gene Array Scanner was used for microarray scanning.
|
| Description |
Ten micron section of OCT-embedded Lateral Geniculate Nucleus was placed on Leica membrane slides.
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) version 1.4 was used for the data processing.
|
| |
|
| Submission date |
Dec 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Henry J. Kaminski |
| E-mail(s) |
henry.kaminski@case.edu, HKaminsk@slu.edu
|
| Phone |
216-368-0250
|
| Fax |
216-368-1710
|
| Organization name |
Case Western Reserve University
|
| Department |
Neurology
|
| Lab |
Henry J. Kaminski(henry.kaminski@case.edu)
|
| Street address |
11100 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106-5068 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE9795 |
Monocular Visual Deprivation in Macaque Monkeys: A Profile in the Gene Expression of the LGN by LCM |
|
