|
Status |
Public on Dec 01, 2008 |
Title |
Ipsilateral LGN layer 5 (deprived layer) of Monocular Deprived for 4 months |
Sample type |
RNA |
|
|
Source name |
Lateral Geniculate Nucleus underwent Monocular Deprived for 4 months
|
Organism |
Macaca mulatta |
Characteristics |
Lateral Geniculate Nucleus were used from female Macaque monkeys using tarsoraphoplasty underwent Monocular Deprived for 4 months.
|
Extracted molecule |
total RNA |
Extraction protocol |
Laser Capture Microdissection was used to cut each individual layer from each Lateral Geniculate Nucleus. Total RNA from each layer was isolated using the PicoPure RNA kit from Arcturus.
|
Label |
biotinylated cRNA
|
Label protocol |
Two rounds of linear T7-based RNA amplification was used utilizing T7 RNA polymerase.
|
|
|
Hybridization protocol |
Standard Affymetrix hybridization protocol was used in an Affymetrix GeneChip Fluidics Station 400.
|
Scan protocol |
Hewlett-Packard Gene Array Scanner was used for microarray scanning.
|
Description |
Ten micron section of OCT-embedded Lateral Geniculate Nucleus was placed on Leica membrane slides.
|
Data processing |
Affymetrix GeneChip Operating Software (GCOS) version 1.4 was used for the data processing.
|
|
|
Submission date |
Dec 05, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Henry J. Kaminski |
E-mail(s) |
henry.kaminski@case.edu, HKaminsk@slu.edu
|
Phone |
216-368-0250
|
Fax |
216-368-1710
|
Organization name |
Case Western Reserve University
|
Department |
Neurology
|
Lab |
Henry J. Kaminski(henry.kaminski@case.edu)
|
Street address |
11100 Euclid Avenue
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44106-5068 |
Country |
USA |
|
|
Platform ID |
GPL3535 |
Series (1) |
GSE9795 |
Monocular Visual Deprivation in Macaque Monkeys: A Profile in the Gene Expression of the LGN by LCM |
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