|
| Status |
Public on Feb 04, 2017 |
| Title |
CON46F |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA from corneal endothelial tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
age: 46
tissue: Control corneal endothelium (CE) tissue
disease status: Healthy
|
| Treatment protocol |
corneal endothelial tissue stored in RNAlater until DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from corneal endothelial tissue using Macherey Nagel NucleoSpin Tissue XS according to manufacturer instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
TEM reagent and Xstaining using Illumina chemistry
|
| |
|
| Hybridization protocol |
Dispense fragmented and resuspended DNA samples onto Illumina Beadchips at 48C overnight in an Illumina Hybridization oven
|
| Scan protocol |
Illumina iScan system
|
| Description |
Control CE sample; Used in final analysis
|
| Data processing |
Raw intensity estimates were imported from the IDAT files and processed using the methylumi R package. We corrected for color biases (adjColorBias.quantile function) and background intensity ("bgAdjust2C" method implemented in lumiMethyB) before quantile normalization (lumiMethyN).
|
| |
|
| Submission date |
Feb 03, 2017 |
| Last update date |
Feb 04, 2017 |
| Contact name |
Selene Clay |
| Organization name |
University of California, San Francisco
|
| Department |
Ophthalmology
|
| Lab |
Matilda Chan
|
| Street address |
1001 Potrero Ave Rm 210
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94110 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE94462 |
Comprehensive characterization of DNA methylation changes in Fuchs Endothelial Corneal Dystrophy |
|
