|
| Status |
Public on Feb 01, 2008 |
| Title |
Brain_ProtocolI_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ambion Human Brain Reference RNA (Catalog #6050)
|
| Organism |
Homo sapiens |
| Characteristics |
Brain_ProtocolI_rep2
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted
|
| Label |
Cy3
|
| Label protocol |
Targets were prepared from 200 ng total RNA using Illumina TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791)
|
| |
|
| Hybridization protocol |
Illumina HumanRef-8 BeadChips were hybridized, washed, stained and scanned according to Illumina’s protocol using the updated wash procedure
|
| Scan protocol |
Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
|
| Description |
Samples labeling and hybridization day 1, replicate 2
|
| Data processing |
Data were extracted with Illumina BeadStudio software using background subtraction and cubic spline normalization. Data were then adjusted by shifting the absolute minimum value for each array to be equal to 1; and then log2 transformed.
|
| |
|
| Submission date |
Dec 07, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
Sylvain.Pradervand@unil.ch
|
| Phone |
+41 21 692 39 08
|
| Organization name |
UNI Lausanne
|
| Department |
CIG
|
| Lab |
DNA Array Facility
|
| Street address |
Genopode
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6255 |
| Series (1) |
| GSE9819 |
Comparisons of Affymetrix Whole-Transcript Human Gene 1.0 ST array with standard 3' expression arrays |
|
