|
| Status |
Public on Mar 31, 2017 |
| Title |
KO_DSS_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse colon epithelial cells, KO, DSS treatment for 2 days
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
genotype/variation: SMS2 KO
cell type: Colon epithelial cells
treatment: 3.5% DSS in drinking water during 2 days
|
| Treatment protocol |
3.5% DSS in drinking water during 2 days.
|
| Growth protocol |
Mice were fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions.
|
| Label |
DLR (Biotin-11-dXTP Analog)
|
| Label protocol |
DLR-labeled single-stranded DNA was synthesized using the Affymetrix GeneChip® WT PLUS Reagent Kit (Affymetrix), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix Clariom™ S Mouse Array by GeneChip® Hybridization Oven 645 according to the protocols recommended by the manufacturer (Affymetrix, Inc).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G (Affymetrix, Inc).
|
| Description |
KO-DSS2
Gene expression data from SMS2 KO mouse colon epithelial cells after 2-days DSS treatment.
|
| Data processing |
The data were analyzed with Affymetrix® Expression Console™ Software (Affymetrix, Inc).
|
| |
|
| Submission date |
Feb 07, 2017 |
| Last update date |
Mar 31, 2017 |
| Contact name |
Chieko Hashizume |
| E-mail(s) |
hashizume.c29@gmail.com
|
| Phone |
+81 76 218 8337
|
| Organization name |
Kanazawa Medical University
|
| Department |
Medicine
|
| Lab |
Hematology/Immunology
|
| Street address |
1-1 Daigaku
|
| City |
Uchinada |
| State/province |
N/A |
| ZIP/Postal code |
9200293 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE94624 |
Colon epithelial cells gene expression data of Sphingomyelin synthase 2 knockout colitis mice |
|
