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Sample GSM250920 Query DataSets for GSM250920
Status Public on Dec 16, 2008
Title apoE-Aorta-6-weeks-3
Sample type RNA
 
Source name total aorta
Organism Mus musculus
Characteristics strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 6 weeks
tissue: aorta
Treatment protocol excised, rinsed with cold PBS (2mM EDTA), removed adipose and connective tissue
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Dec 17, 2007
Last update date Aug 28, 2018
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (2)
GSE10000 Age-dependent aorta transcriptomes in wild-type and apoE-deficient C57BL/6J mice
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 378.7 P 0.006871
AFFX-BioB-M_at 459.8 P 0.00034
AFFX-BioB-3_at 220.9 P 0.001248
AFFX-BioC-5_at 1111.8 P 0.000127
AFFX-BioC-3_at 1099.8 P 0.000044
AFFX-BioDn-5_at 2098.4 P 0.00006
AFFX-BioDn-3_at 4747.6 P 0.000169
AFFX-CreX-5_at 10006.8 P 0.000052
AFFX-CreX-3_at 10622.4 P 0.000044
AFFX-DapX-5_at 725.9 P 0.00007
AFFX-DapX-M_at 1088.3 P 0.001102
AFFX-DapX-3_at 1260.1 P 0.00007
AFFX-LysX-5_at 96.2 P 0.000857
AFFX-LysX-M_at 212.7 M 0.062929
AFFX-LysX-3_at 342.1 P 0.000095
AFFX-PheX-5_at 107.7 P 0.010317
AFFX-PheX-M_at 154.1 P 0.026111
AFFX-PheX-3_at 187.5 P 0.005565
AFFX-ThrX-5_at 118.3 P 0.009337
AFFX-ThrX-M_at 229.5 P 0.000258

Total number of rows: 45101

Table truncated, full table size 1223 Kbytes.




Supplementary file Size Download File type/resource
GSM250920.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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