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Sample GSM250943 Query DataSets for GSM250943
Status Public on Dec 16, 2008
Title apoE-Aorta-78-weeks-3
Sample type RNA
 
Source name total aorta
Organism Mus musculus
Characteristics strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 78 weeks
tissue: aorta
Treatment protocol excised, rinsed with cold PBS (2mM EDTA), removed adipose and connective tissue
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Dec 17, 2007
Last update date Aug 28, 2018
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (2)
GSE10000 Age-dependent aorta transcriptomes in wild-type and apoE-deficient C57BL/6J mice
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 324.2 P 0.002867
AFFX-BioB-M_at 520.5 P 0.000225
AFFX-BioB-3_at 248.3 P 0.000081
AFFX-BioC-5_at 1041.7 P 0.00011
AFFX-BioC-3_at 1252.1 P 0.000044
AFFX-BioDn-5_at 1858.1 P 0.000052
AFFX-BioDn-3_at 5037.1 P 0.000147
AFFX-CreX-5_at 10164.1 P 0.000052
AFFX-CreX-3_at 12971 P 0.000044
AFFX-DapX-5_at 118.2 P 0.001593
AFFX-DapX-M_at 298.6 P 0.006871
AFFX-DapX-3_at 272.6 P 0.000972
AFFX-LysX-5_at 21.9 A 0.195266
AFFX-LysX-M_at 61 A 0.48511
AFFX-LysX-3_at 65.1 P 0.002867
AFFX-PheX-5_at 32.4 A 0.5
AFFX-PheX-M_at 30.9 A 0.354453
AFFX-PheX-3_at 64.1 A 0.165861
AFFX-ThrX-5_at 45 A 0.262827
AFFX-ThrX-M_at 85.1 A 0.123572

Total number of rows: 45101

Table truncated, full table size 1220 Kbytes.




Supplementary file Size Download File type/resource
GSM250943.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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