|
| Status |
Public on Feb 22, 2018 |
| Title |
SKBR3 Cells treated with CAF15-CM rep2 |
| Sample type |
genomic |
| |
|
| Source name |
SKBR3_CAF15-CM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKBR3
treated with: CAF15-CM
|
| Treatment protocol |
SKBR3 and AU565 were treated by CAF-CMs or DAC as specified in the methods section
|
| Growth protocol |
SKBR3 and AU565 cell lines were cultivated as the manufacturer’s instructions (ATCC).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from cell lines using the QIAmp DNA Mini Kit (Qiagen)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
SKBR3_CAF15-CM
SK-CAF15-2
|
| Data processing |
Genome Studio V2011.2
Signal_A and Signal_B intensities in idat files
|
| |
|
| Submission date |
Feb 27, 2017 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Robert Dante |
| E-mail(s) |
robert.dante@lyon.unicaner.fr
|
| Phone |
+33478785922
|
| Organization name |
Cancer Research Center – Lyon
|
| Department |
Department of Tumoral Escape
|
| Street address |
28, rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69373 Lyon Cedex 08 |
| Country |
France |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE95460 |
DNA methylation signature defines genes modulated by stromal cell contents of human breast tumors [MethylationEPIC] |
| GSE95462 |
DNA methylation signature defines genes modulated by stromal cell contents of human breast tumors |
|
