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Sample GSM25155 Query DataSets for GSM25155
Status Public on Jun 24, 2004
Title PGA-E11.5_3
Sample type RNA
Source name Whole heart
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 11.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 238.2 P 0.005565
AFFX-BioB-M_at 326.2 P 0.00034
AFFX-BioB-3_at 135.1 P 0.001248
AFFX-BioC-5_at 630.8 P 0.000147
AFFX-BioC-3_at 625.1 P 0.000044
AFFX-BioDn-5_at 862.8 P 0.00007
AFFX-BioDn-3_at 3966.5 P 0.00011
AFFX-CreX-5_at 9400.9 P 0.000052
AFFX-CreX-3_at 9309.6 P 0.000044
AFFX-DapX-5_at 14.1 A 0.48511
AFFX-DapX-M_at 38.4 A 0.275146
AFFX-DapX-3_at 29 A 0.645547
AFFX-LysX-5_at 21.6 A 0.227636
AFFX-LysX-M_at 14.1 A 0.724854
AFFX-LysX-3_at 29.6 A 0.102165
AFFX-PheX-5_at 4 A 0.9273
AFFX-PheX-M_at 3.9 A 0.814869
AFFX-PheX-3_at 13.8 A 0.672921
AFFX-ThrX-5_at 18.5 A 0.5
AFFX-ThrX-M_at 5.7 A 0.724854

Total number of rows: 45101

Table truncated, full table size 1221 Kbytes.

Supplementary file Size Download File type/resource
GSM25155.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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