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Sample GSM25168 Query DataSets for GSM25168
Status Public on Jun 24, 2004
Title PGA-E14.5_1
Sample type RNA
Source name Both ventricles
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 14.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 245.5 P 0.033677
AFFX-BioB-M_at 191.2 P 0.001593
AFFX-BioB-3_at 140.9 A 0.0727
AFFX-BioC-5_at 411.6 P 0.000662
AFFX-BioC-3_at 329.7 P 0.001593
AFFX-BioDn-5_at 542.1 P 0.001593
AFFX-BioDn-3_at 3993.7 P 0.000662
AFFX-CreX-5_at 7754 P 0.00007
AFFX-CreX-3_at 9196.7 P 0.000044
AFFX-DapX-5_at 10.9 A 0.354453
AFFX-DapX-M_at 31.1 A 0.425962
AFFX-DapX-3_at 14.7 A 0.868639
AFFX-LysX-5_at 7.8 A 0.574038
AFFX-LysX-M_at 60.9 A 0.574038
AFFX-LysX-3_at 48 A 0.131361
AFFX-PheX-5_at 7.6 A 0.910522
AFFX-PheX-M_at 3.7 A 0.945787
AFFX-PheX-3_at 36.3 A 0.60308
AFFX-ThrX-5_at 24.8 A 0.645547
AFFX-ThrX-M_at 23.5 A 0.51489

Total number of rows: 45101

Table truncated, full table size 1224 Kbytes.

Supplementary file Size Download File type/resource
GSM25168.CEL.gz 6.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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