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Sample GSM25169 Query DataSets for GSM25169
Status Public on Jun 24, 2004
Title PGA-E14.5_2
Sample type RNA
Source name Both ventricles
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 14.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 278.3 P 0.015183
AFFX-BioB-M_at 332 P 0.000754
AFFX-BioB-3_at 126.2 P 0.039661
AFFX-BioC-5_at 648.3 P 0.000258
AFFX-BioC-3_at 537.7 P 0.00011
AFFX-BioDn-5_at 895.3 P 0.00007
AFFX-BioDn-3_at 3522.4 P 0.000446
AFFX-CreX-5_at 9679.9 P 0.000052
AFFX-CreX-3_at 8434.9 P 0.000044
AFFX-DapX-5_at 13.8 A 0.41138
AFFX-DapX-M_at 57.7 A 0.195266
AFFX-DapX-3_at 16.4 A 0.699394
AFFX-LysX-5_at 5.9 A 0.760937
AFFX-LysX-M_at 10.4 A 0.860518
AFFX-LysX-3_at 33.5 A 0.313723
AFFX-PheX-5_at 2.4 A 0.960339
AFFX-PheX-M_at 2.8 A 0.969024
AFFX-PheX-3_at 16.8 A 0.760937
AFFX-ThrX-5_at 14.1 A 0.712257
AFFX-ThrX-M_at 25.2 A 0.5

Total number of rows: 45101

Table truncated, full table size 1223 Kbytes.

Supplementary file Size Download File type/resource
GSM25169.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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