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Sample GSM25171 Query DataSets for GSM25171
Status Public on Jun 24, 2004
Title PGA-E14.5_1A
Sample type RNA
Source name Atrial chamber of heart
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 14.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 248.2 P 0.005565
AFFX-BioB-M_at 325.1 P 0.000297
AFFX-BioB-3_at 124.8 P 0.021902
AFFX-BioC-5_at 483.9 P 0.000225
AFFX-BioC-3_at 455.1 P 0.00007
AFFX-BioDn-5_at 738.4 P 0.00007
AFFX-BioDn-3_at 3072.2 P 0.00034
AFFX-CreX-5_at 7600.2 P 0.000052
AFFX-CreX-3_at 7737.2 P 0.000044
AFFX-DapX-5_at 28.8 A 0.131361
AFFX-DapX-M_at 13.8 A 0.574038
AFFX-DapX-3_at 7.3 A 0.794268
AFFX-LysX-5_at 30.1 M 0.050229
AFFX-LysX-M_at 7.2 A 0.783476
AFFX-LysX-3_at 17.6 A 0.147939
AFFX-PheX-5_at 2.1 A 0.966323
AFFX-PheX-M_at 2 A 0.973889
AFFX-PheX-3_at 42.7 A 0.313723
AFFX-ThrX-5_at 12.3 A 0.58862
AFFX-ThrX-M_at 20.7 A 0.340661

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM25171.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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