GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM25173 Query DataSets for GSM25173
Status Public on Jun 24, 2004
Title PGA-E14.5_3A
Sample type RNA
Source name Atrial chamber of heart
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 14.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 190.2 P 0.002556
AFFX-BioB-M_at 447.7 P 0.000147
AFFX-BioB-3_at 139 P 0.023929
AFFX-BioC-5_at 573 P 0.000147
AFFX-BioC-3_at 492.1 P 0.000052
AFFX-BioDn-5_at 741 P 0.00011
AFFX-BioDn-3_at 3946.1 P 0.000195
AFFX-CreX-5_at 7566 P 0.000052
AFFX-CreX-3_at 9003.2 P 0.000044
AFFX-DapX-5_at 20.2 A 0.300606
AFFX-DapX-M_at 28.3 A 0.382599
AFFX-DapX-3_at 15.1 A 0.574038
AFFX-LysX-5_at 8.7 A 0.455413
AFFX-LysX-M_at 11.3 A 0.814869
AFFX-LysX-3_at 35.8 A 0.156732
AFFX-PheX-5_at 5.4 A 0.876428
AFFX-PheX-M_at 2.4 A 0.949771
AFFX-PheX-3_at 33.6 A 0.39692
AFFX-ThrX-5_at 9.6 A 0.834139
AFFX-ThrX-M_at 5.6 A 0.5

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM25173.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap