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Sample GSM25181 Query DataSets for GSM25181
Status Public on Jun 24, 2004
Title PGA-E18.5_2
Sample type RNA
Source name Both ventricles
Organism Mus musculus
Extracted molecule total RNA
Description C57BL/6 benchmark dataset: Embryonic day 18.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Reanalyzed by GSE119085

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 320.8 P 0.000857
AFFX-BioB-M_at 533.4 P 0.000147
AFFX-BioB-3_at 290.3 P 0.000225
AFFX-BioC-5_at 840.5 P 0.000127
AFFX-BioC-3_at 925.4 P 0.000052
AFFX-BioDn-5_at 1191 P 0.000052
AFFX-BioDn-3_at 5015.4 P 0.000297
AFFX-CreX-5_at 13350.3 P 0.000052
AFFX-CreX-3_at 12284.1 P 0.000044
AFFX-DapX-5_at 24.1 A 0.275146
AFFX-DapX-M_at 30.3 A 0.123572
AFFX-DapX-3_at 8.2 A 0.876428
AFFX-LysX-5_at 29.4 A 0.205732
AFFX-LysX-M_at 48.2 A 0.58862
AFFX-LysX-3_at 21.3 A 0.175328
AFFX-PheX-5_at 2.5 A 0.941556
AFFX-PheX-M_at 3.2 A 0.897835
AFFX-PheX-3_at 39.4 A 0.48511
AFFX-ThrX-5_at 6.7 A 0.804734
AFFX-ThrX-M_at 23 A 0.41138

Total number of rows: 45101

Table truncated, full table size 1219 Kbytes.

Supplementary file Size Download File type/resource
GSM25181.CEL.gz 6.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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