NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM25184 Query DataSets for GSM25184
Status Public on Jun 24, 2004
Title PGA-E18.5_2A
Sample type RNA
 
Source name Atrial chamber of heart
Organism Mus musculus
Extracted molecule total RNA
 
Description C57BL/6 benchmark dataset: Embryonic day 18.5. The most common congenital cardiac abnormalities are associated with the defects in cardiac septation and outflow tract development. Specifically, atrial septal defects (ASDs) account for about one third of the cases detected in adults. To address this question it is imperative to get insight into the genes expressed during normal heart development. Timed matings were performed using C57BL/6 mice and pregnant females sacrificed starting at embryonic day (E) 10.5, and then in daily intervals until E14.5. These developmental stages have been selected as they encompass stages of septation of the atrial and ventricular chambers as well as the cardiac outflow tract. Gene expression was further analyzed at E16.5 and E18.5, to monitor changes in gene expression related to maturation of the heart. At stages 10.5 and 11.5, we have removed the rostral and caudal parts of the embryo and subjected the middle part, which includes the heart, for expression analysis. From embryonic day 12.5 on, we isolated embryonic hearts and separated the ventricular from the atrial chambers.
Keywords = cardiac development, heart
 
Submission date Jun 15, 2004
Last update date Aug 28, 2018
Contact name Martina Schinke
URL http://www.cardiogenomics.org
Organization name Harvard University
Department Bauer Center for Genomic Research
Lab Cardiogenomics
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL1261
Series (1)
GSE1479 C57BL/6 Benchmark Set for Early Cardiac Development
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 247.2 P 0.005565
AFFX-BioB-M_at 276.6 P 0.000509
AFFX-BioB-3_at 110.6 M 0.054213
AFFX-BioC-5_at 472.2 P 0.000258
AFFX-BioC-3_at 453.3 P 0.00011
AFFX-BioDn-5_at 552.5 P 0.000095
AFFX-BioDn-3_at 2991 P 0.00034
AFFX-CreX-5_at 6815 P 0.000052
AFFX-CreX-3_at 7137.5 P 0.000044
AFFX-DapX-5_at 29.4 A 0.131361
AFFX-DapX-M_at 7 A 0.544587
AFFX-DapX-3_at 23.5 A 0.574038
AFFX-LysX-5_at 7.1 A 0.544587
AFFX-LysX-M_at 7.4 A 0.699394
AFFX-LysX-3_at 21.6 A 0.139482
AFFX-PheX-5_at 1.7 A 0.981719
AFFX-PheX-M_at 2 A 0.937071
AFFX-PheX-3_at 30 A 0.470241
AFFX-ThrX-5_at 15.7 A 0.686277
AFFX-ThrX-M_at 2.5 A 0.772364

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.




Supplementary file Size Download File type/resource
GSM25184.CEL.gz 6.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap