|
| Status |
Public on Sep 12, 2018 |
| Title |
E9.5_Dgcr8-cKO+miR-541_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
E9.5_Dgcr8-cKO+miR-541
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Mesp1Cre/+/Dgcr8-/-
age: Embryonic day 9.5
transfected with: miR-541 mimics
cell type: cultured heart cells
|
| Treatment protocol |
In vitro cultured Dgcr8 cKO heart cells were transfected with miR-541-5p and NC miRNA 24 hrs after plating, and total RNA were collected 48 hrs after transfection
|
| Growth protocol |
Dgcr8 cKO heart cells were dissociated into very small clusters, and cultured in vitro in a chemical defined medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIZOL and treated with RNase free DNase.
RNA library was prepared following the instruction of SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing（Clontech）
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
cultured heart cells
|
| Data processing |
Sequenced reads were trimmed for adaptor sequences, and masked for low-complexity or low-quality sequences, then mapped to mm10 genome using STAR.
Fragments Per Kilobase per Million mapped reads (FPKM) of Refseq genes were caculated.
Genome_build: mm10
Supplementary_files_format_and_content: Excel files of gene names and FPKM values for each sample.
|
| |
|
| Submission date |
Mar 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jie Na |
| E-mail(s) |
jie.na@tsinghua.edu.cn
|
| Phone |
+86-18618192150
|
| Organization name |
Tsinghua University
|
| Department |
Basic Medical Sciences, School of Medicine
|
| Lab |
D115 School of Medicine
|
| Street address |
Tsinghua University
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE96647 |
Next Generation Sequencing Analysis of gene expression profile in E9.5 Mesp1Cre/+/Dgcr8-/- embryonic heart cells transfected with NC miRNA and miR-541 mimics |
| GSE96648 |
Transcriptome analysis of mouse primitive heart with Dgcr8 deletion |
|
| Relations |
| BioSample |
SAMN06603938 |
| SRA |
SRX2642195 |
