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Sample GSM254298 Query DataSets for GSM254298
Status Public on Nov 03, 2008
Title Untreated MCF7 RNA-2
Sample type RNA
 
Source name MCF7,untreated
Organism Homo sapiens
Characteristics ER+/PR+
Growth protocol Cells are maintained routinely at 37C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 10ng/ml insulin prior to treatments
Extracted molecule total RNA
Extraction protocol Qiagen RNAesy purification
Label biotin
Label protocol Affymetrix Gene Expression TP 21 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
 
Hybridization protocol Affymetrix Comm Hyb Setup 21 & Affymetrix Comm Wash Stain 21 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
Scan protocol GeneChip HT Scanner protocol v2.2.09 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
Description Gene expression data from untreated MCF7
Data processing RMA normalization with affy package in Bioconductor R
 
Submission date Jan 03, 2008
Last update date Jan 19, 2010
Contact name Christina Yau
E-mail(s) cyau@buckinstitute.org
Phone 415-209-2248
Organization name Buck Institute of Age Research
Lab Benz Lab
Street address 8001 Redwood Blvd
City Novato
State/province CA
ZIP/Postal code 94945
Country USA
 
Platform ID GPL3921
Series (1)
GSE10061 Expression data of MCF7 cells following short-term E deprivation, ER-alpha siRNA knockdown and oxidant treatments

Data table header descriptions
ID_REF
VALUE RMA normalized log2 intensity

Data table
ID_REF VALUE
1007_s_at 10.34717781
1053_at 10.71912676
117_at 5.398105278
121_at 8.336374627
1255_g_at 4.971768281
1294_at 5.82535356
1316_at 5.293236596
1320_at 6.248402756
1405_i_at 4.778605701
1431_at 5.268064835
1438_at 6.662120824
1487_at 8.548576046
1494_f_at 5.945838884
1598_g_at 7.682803585
160020_at 6.439368418
1729_at 8.312179663
177_at 5.848817292
1773_at 8.099487668
179_at 8.678592002
1861_at 8.443867145

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM254298.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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