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Sample GSM254428 Query DataSets for GSM254428
Status Public on May 08, 2008
Title GM9016, affected brother
Sample type RNA
 
Source name GM9016
Organism Homo sapiens
Characteristics affected brother
Biomaterial provider Corielle
Growth protocol B-Cells from Corielle were incubated at 37oC and 5% CO2 overnight, the day after cells are counted. Cells were expanded in RPMI 1640 with 2mM L-glutamine and 15% FBS following Corielle's lymphoblast line maintenance protocols. 1x10^7 cells were spun down and resuspended in 2 ml Tri-Reagent and frozen overnight at -80oC prior to RNA extraction
Extracted molecule total RNA
Extraction protocol Std Tri-Reagent protocol followed by cleanup on RNEasy columns using an on-column DNase step. RNA quality is checked using a Nanodrop and an Agilent Bioanalyzer
Label biotin-11-UTP
Label protocol Standard Codelink protocol
 
Hybridization protocol Standard Codelink protocol
Scan protocol Standard Codelink protocol on Axon genePix 4000B
Description GM9016, affected brother, 38yrs, family 2112
Data processing Codelink Expression Analysis Softwarev4.2
 
Submission date Jan 03, 2008
Last update date May 08, 2008
Contact name Sridar V Chittur
E-mail(s) schittur@albany.edu
Phone 518-591-7215
Organization name SUNY-University at Albany
Department Center for Functional Genomics
Lab Microarray Core
Street address One Discovery Drive, CRC 342G
City Rensselaer
State/province NY
ZIP/Postal code 12144
Country USA
 
Platform ID GPL2895
Series (1)
GSE10064 Gene expression in immortalized B-lymphocytes derived from patients with multiple sclerosis

Data table header descriptions
ID_REF
VALUE NORMALIZED EXPRESSION VALUE
EXPRESSIONVALUE RAW EXPRESSION VALUE
GENESPRINGFLAG P=Present, A=Absent
CODELINKFLAG L=Low signal, G=Good signal
SPOT_COL Spot column
SPOT_ROW Spot row
SPOTMEAN
SPOTMEDIAN
BKGMEAN
BKGMEDIAN

Data table
ID_REF VALUE EXPRESSIONVALUE GENESPRINGFLAG CODELINKFLAG SPOT_COL SPOT_ROW SPOTMEAN SPOTMEDIAN BKGMEAN BKGMEDIAN
1002 6.811767106 361.5866699 P G 2 1 408.5866699 430 48.64640808 47
1003 0.129921032 6.89655304 A L 3 1 58.89655304 58 54.73480606 52
1004 0.302077735 16.03508759 A L 4 1 64.03508759 60 53.98039246 48
1005 0.117685506 6.247058868 A L 5 1 60.24705887 54 55.94083023 54
1006 0.240604604 12.77193069 A L 6 1 61.77193069 59 52.1696434 49
1007 0.187831393 9.970588684 A L 7 1 62.97058868 62 55.80701828 53
1009 1.039608707 55.18518829 P G 9 1 108.1851883 117 56.58241653 53
1010 6.071434901 322.2878723 P G 10 1 371.2878723 391 54.5549469 49
1011 0.14466581 7.679244995 A L 11 1 61.679245 57 56.30508423 54
1012 0.293006712 15.55357361 A L 12 1 67.55357361 63 52.81000137 52
1013 0.147383897 7.82352829 A L 13 1 58.82352829 55 58.18796921 51
1014 0.262206224 13.91860199 A L 14 1 65.91860199 58 54.52252197 52
1016 0.307892486 16.34375 A L 16 1 72.34375 67 59.20833206 56
1017 0.028846524 1.53125 A L 17 1 56.53125 53 56.808918 55
1018 0.297770533 15.80644989 A L 18 1 68.80644989 64 56.90110016 53
1019 -0.026164655 -1.388889313 A L 19 1 53.61111069 49 59.19166565 55
1020 0.110017111 5.840000153 A L 20 1 58.84000015 54 55.8125 53
1021 6.389816561 339.1884155 P G 21 1 392.1884155 430 55.57926941 53
1023 3.975994479 211.0563354 P G 23 1 263.0563354 251 53.33734894 52
1024 0.059153023 3.13999939 A L 24 1 48.13999939 45 49.7964592 45

Total number of rows: 54167

Table truncated, full table size 3748 Kbytes.




Supplementary file Size Download File type/resource
GSM254428.tif.gz 44.4 Mb (ftp)(http) TIFF
Processed data included within Sample table

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