|
| Status |
Public on Oct 10, 2009 |
| Title |
PBMC_CD3CD28Activated_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC CD3 CD28 Stimulated
|
| Organism |
Homo sapiens |
| Characteristics |
Cryopreserved PBMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard TRIZOL Extraction followed by Qiagen RNeasy Column Purification
|
| Label |
Cy3
|
| Label protocol |
250ng Total RNA amplified and labelled according to Ambion Illumina TotalPrep RNA Amplification Kit protocol
|
| |
|
| Hybridization protocol |
1.5ug labelled cRNA Hybridized according to Illumina Standard protocol
|
| Scan protocol |
Scanned on Illumina BeadStation
|
| Description |
Replicate 3 of 6
|
| Data processing |
Data quantile normalised on Illumina BeadStudio Gene Expression Module v3.2.3
|
| |
|
| Submission date |
Jan 07, 2008 |
| Last update date |
Oct 22, 2008 |
| Contact name |
Graham Heap |
| E-mail(s) |
g.heap@qmul.ac.uk
|
| Organization name |
QMUL
|
| Street address |
4 Newark Street
|
| City |
London |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE10080 |
IL-23 100ng/ml Treatment of CD3 CD28 Stimulated PBMCs |
|
