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Sample GSM257240 Query DataSets for GSM257240
Status Public on Mar 15, 2008
Title BMDM_unpretreated-MDP_3h_rep2
Sample type RNA
 
Source name Mus musculus bone marrow derived macrophages
Organism Mus musculus
Characteristics C57BL/6
Treatment protocol BMDMs were left untreated for 24 h and stimulated with 10 mg/ml MDP for 3 h
Extracted molecule total RNA
Extraction protocol Total RNA was purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip scanner 3000 7G.
Description Gene expression data from bone marrow derived macrophages
Data processing The data were analyzed using the affy package of Bioconductor. Raw probe-level data were converted to expression measures using the Robust Multi-array Average (RMA) method.
 
Submission date Jan 15, 2008
Last update date Aug 02, 2019
Contact name Yun-Gi Kim
E-mail(s) yungikim@umich.edu
Organization name University of Michigan
Department Pathology
Lab Gabriel Nunez
Street address 1500 E. Medical Center Dr. 4111 CCGC
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL1261
Series (1)
GSE10182 MDP- and Pam3CSK4-induced genes in naïve and tolerant macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE RMA(Robust Multi-array Average)-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 9.574477609
1415671_at 12.78052517
1415672_at 12.28527607
1415673_at 7.466523595
1415674_a_at 10.79952114
1415675_at 10.5036672
1415676_a_at 12.32023811
1415677_at 11.02534603
1415678_at 10.8592984
1415679_at 12.82235946
1415680_at 9.413590474
1415681_at 10.85660959
1415682_at 8.108731297
1415683_at 11.73507651
1415684_at 9.10651216
1415685_at 9.448579816
1415686_at 10.41088684
1415687_a_at 14.49419846
1415688_at 10.75167211
1415689_s_at 8.419100115

Total number of rows: 45101

Table truncated, full table size 1025 Kbytes.




Supplementary file Size Download File type/resource
GSM257240.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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