|
Status |
Public on Mar 15, 2008 |
Title |
BMDM_unpretreated-MDP_3h_rep2 |
Sample type |
RNA |
|
|
Source name |
Mus musculus bone marrow derived macrophages
|
Organism |
Mus musculus |
Characteristics |
C57BL/6
|
Treatment protocol |
BMDMs were left untreated for 24 h and stimulated with 10 mg/ml MDP for 3 h
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified with RNeasy Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430 2.0.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 7G.
|
Description |
Gene expression data from bone marrow derived macrophages
|
Data processing |
The data were analyzed using the affy package of Bioconductor. Raw probe-level data were converted to expression measures using the Robust Multi-array Average (RMA) method.
|
|
|
Submission date |
Jan 15, 2008 |
Last update date |
Aug 02, 2019 |
Contact name |
Yun-Gi Kim |
E-mail(s) |
yungikim@umich.edu
|
Organization name |
University of Michigan
|
Department |
Pathology
|
Lab |
Gabriel Nunez
|
Street address |
1500 E. Medical Center Dr. 4111 CCGC
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE10182 |
MDP- and Pam3CSK4-induced genes in naïve and tolerant macrophages |
|
Relations |
Reanalyzed by |
GSE119085 |
Reanalyzed by |
GSE135324 |