|
| Status |
Public on Apr 11, 2017 |
| Title |
Mutant_175 |
| Sample type |
RNA |
| |
|
| Source name |
heart Tg + Tamoxifen
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Brg1 and Brm knockout
age: adult
gender: female
cell type: cardiomyocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Norgen #25700 Animal Tissue RNA Purification Kit following manufacturers specifications.
|
| Label |
Cy3
|
| Label protocol |
Agilent Low Input Quick Amp Labeling Protocol for Cy3 labeling. In tube add RNA, spike mix and T7 primer. Denature. Add cDNA master mix including Affinity Script RNAse Block Mix. Incubate. Add Transcription master mix including T7 RNA Polymerase blend and incubate, then purify labeled/amplified RNA & quantify.
|
| |
|
| Hybridization protocol |
Agilent's Hybridization Oven set at 65C, Add Cy3 labeled cRNA, gene expression blocking agent and fragmentation buffer. Incubate. Add HI-RPM Hybridization buffer to stop reaction. Use hybridization chamber , slides with barcodes,. Load samples and hybridize rotating in chamber 17 hrs. Wash in 2 buffers, and then Milli Q water wash
|
| Scan protocol |
Agilent C Microarray Scanner, Feature Extraction software 10.7.1 or later, Agilent Scan Control software.
|
| Data processing |
Agilent feature extraction software.
|
| |
|
| Submission date |
Apr 10, 2017 |
| Last update date |
Apr 11, 2017 |
| Contact name |
Scott Bultman |
| E-mail(s) |
scott_bultman@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Department of Pathology and Laboratory Medicine; Department of Genetics
|
| Lab |
Bultman Lab
|
| Street address |
4345 MBRB - CB#7264
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE97593 |
Gene expression changes in adult mouse cardiomyocytes when Brg1 and Brm are knocked out |
|
