|
| Status |
Public on Apr 15, 2017 |
| Title |
U251 cells transfected with Scr-shRNA, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
U251 cells transfected with Scr-shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U251
|
| Treatment protocol |
BRD4-shRNA-transfected and Scr-shRNA-transfected U251 cells were rinsed with PBS and placed on ice in the Trizol solution (Life Technologies).
|
| Growth protocol |
BRD4-shRNA-transfected and Scr-shRNA-transfected U251 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Clariom®D Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
|
| Data processing |
The data were analyzed with affmetrix expression console default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 14, 2017 |
| Last update date |
Apr 15, 2017 |
| Contact name |
Feng jun lin |
| E-mail(s) |
tangweiping2@126.com
|
| Phone |
15845412541
|
| Organization name |
1Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education
|
| Department |
Department of Pathophysiology
|
| Lab |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
2Institute of Health Sciences
|
| City |
Shanghai, |
| State/province |
xvhuiqv |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE97791 |
Analysis of BRD4-regulated genes in human glioma U251 cells |
|
