|
| Status |
Public on Apr 14, 2021 |
| Title |
CochlearLateralWall_YoungAdult_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Cochlear lateral wall, young adult
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cochlear lateral wall
age: 1-3 month
age type: young
|
| Treatment protocol |
CBA/CaJ young adult (1-3 months) and aged (1.5 years) mice were used in this study. Mice were euthanized and CLW tissues were micro-dissected from the inner ear. All sample types were prepared in experimental triplicate (n=3).
|
| Growth protocol |
CBA/CaJ mice were bred in-house in a low-noise environment at the Animal Research Facility of the Medical University of South Carolina (MUSC) with original breeding pairs purchased from The Jackson Laboratory (Bar Harbor, ME). All mice received food and water ad libitum and were maintained on a 12-hour light/dark cycle. Mice of both genders were used in the study. All aspects of the animal research were conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee of the MUSC. Prior to treatment, mice were examined for signs of external ear canal and middle ear obstruction, and mice exhibiting these signs were excluded from the study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Microdissected CLW underwent total RNA preparation using the miRNeasy Minikit (Qiagen). RNA preparations were run on an Agilent 2100 Bioanalyzer to ensure that total RNA samples retained small molecular weight RNA and were of sufficient quality for transcripomic analysis.
|
| Label |
biotin
|
| Label protocol |
Total RNA (100 ng) was converted into biotin-labeled cRNA and then fragmented using the Affymetrix 3' IVT Express Kit in accordance with manufacturer protocols.
|
| |
|
| Hybridization protocol |
Targets (12.5 ug) were hybridized to Affymetrix Mouse 430 2.0 GeneChips according to manufacturer protocols. Post hybridization washing and fluorescence labeling was done in an Affymetrix Fluidics Station 450 in accordance with manufacturer protocols.
|
| Scan protocol |
Microarrays were scanned using an Affymetrix 7G Scanner.
|
| Description |
YLW3_mRNA
|
| Data processing |
CEL files imported into Affymetrix Expression Console software and data were normalized by: 1) RMA to obtain probe set expression values, and 2) MAS5 to obtain probe set detection calls.
|
| |
|
| Submission date |
Apr 21, 2017 |
| Last update date |
Jan 27, 2022 |
| Contact name |
Jeremy Barth |
| E-mail(s) |
barthj@musc.edu
|
| Organization name |
MUSC
|
| Street address |
173 Ashley Ave, BSB 627
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425-5080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE98070 |
MicroRNAs as Regulators of Aging Processes in the Cochlear Lateral Wall of the Mouse Inner Ear (mRNA data set) |
| GSE98072 |
MicroRNAs as Regulators of Aging Processes in the Cochlear Lateral Wall of the Mouse Inner Ear |
|
