|
| Status |
Public on Apr 30, 2008 |
| Title |
HCT116_miR34b |
| Sample type |
RNA |
| |
|
| Source name |
Human colorectal cancer cell line HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
HCT116 transfected with pre-miR34b
|
| Treatment protocol |
HCT116 cells were transfected with 100 pmol of Pre-miR miRNA34b Precursor Molecules (Ambion) using a Cell Line Nucleofector kit V (Amaxa) with a Nucleofector I electroporation device (Amaxa) according to the manufacturer’s instructions. Total RNA was extracted 48 h after transfection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol and RNeasy Mini Elute Cleanup kit
|
| Label |
Cy3
|
| Label protocol |
Agilent protocol
|
| |
|
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Agilent scanner and Agilent scan control software
|
| Description |
Human colorectal cancer cell line with pre-miR34b
|
| Data processing |
Feature Extraction ver 9.5
|
| |
|
| Submission date |
Jan 27, 2008 |
| Last update date |
Feb 11, 2008 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE10455 |
Genes regulated by miR-34b/c and DAC |
|
