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Sample GSM259916 Query DataSets for GSM259916
Status Public on Jan 29, 2008
Title Control IgG for 4 days replicate 2
Sample type RNA
Source name Testis from Sprague Dawley male rat 172, Day 18 postnatal, control antibody for 4 days
Organism Rattus norvegicus
Characteristics male rat 172
Testis dissected from P18-day old Sprague Dawley rats
control antibody for 4 days
Treatment protocol Male day P18 rat were injected with control IgG for 4 days. Day P22 rat were euthanized and testis were removed according to IACUC-approved animal use protocols.
Extracted molecule total RNA
Extraction protocol About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Label biotin
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol chips were scanned using Agilent GeneArray Scanner G2500A
Description Chip ID=ingrid-172-U34A-010903. RNA from P18 day old rat testis.
Data processing Auto-scale ON (1-1000). Scaling = all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Submission date Jan 28, 2008
Last update date Jan 28, 2008
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platform ID GPL85
Series (1)
GSE10293 Rat expression data after acute FSH suppression

Data table header descriptions
ID_REF Affymetrix Probe set ID
VALUE GCOS-calculated signal intensities
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE t-test p-value

Data table
A01157cds_s_at 18.6 A 0.47785076
A03913cds_s_at 16.8 A 0.64161587
A04674cds_s_at 18.7 A 0.43121108
A07543cds_s_at 22.3 A 0.3700223
A09811cds_s_at 389.3 P 0.84437203
A16585cds_s_at 12.2 A 0.5167142
A17753cds_s_at 2.3 A 0.34113944
A30543cds_s_at 5.2 A 0.3973418
A44407cds_at 3.7 A 0.3517835
AA108277_at 89.4 P 0.18021917
AA108308_i_at 0.6 A 0.3460462
AA108308_s_at 140.6 P 0.8071082
AA684537_at 129.1 P 0.3540783
AA684929_f_at 113.8 P 0.8213299
AA684960_f_at 67.7 A 0.49838006
AA684963_at 306.1 P 0.935153
AA685112_at 324.8 P 0.5333168
AA685152_r_at 37.8 P 0.81468254
AA685376_f_at 12.2 A 0.84735745
AA685876_at 157.5 A 0.2868933

Total number of rows: 8799

Table truncated, full table size 263 Kbytes.

Supplementary file Size Download File type/resource
GSM259916.CEL.gz 2.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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