|
| Status |
Public on Jan 29, 2008 |
| Title |
FSH antibody for 4 days replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Testis from Sprague Dawley male rat 161, Day 18 postnatal, FSH antibody for 4 days
|
| Organism |
Rattus norvegicus |
| Characteristics |
male rat 161
Testis dissected from P18-day old Sprague Dawley rats
FSH antibody for 4 days
|
| Treatment protocol |
Male day P18 rat were injected with anti-FSH for 4 days. Day P22 rat were euthanized and testis were removed according to IACUC-approved animal use protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
chips were scanned using Agilent GeneArray Scanner G2500A
|
| Description |
Chip ID=ingrid-161-U34A-010903. RNA from P18 day old rat testis.
|
| Data processing |
Auto-scale ON (1-1000). Scaling = all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
|
| |
|
| Submission date |
Jan 28, 2008 |
| Last update date |
Jan 28, 2008 |
| Contact name |
Michael K Skinner |
| E-mail(s) |
skinner@mail.wsu.edu
|
| Organization name |
WSU
|
| Department |
SBS
|
| Street address |
Abelson 507
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99163 |
| Country |
USA |
| |
|
| Platform ID |
GPL85 |
| Series (1) |
| GSE10293 |
Rat expression data after acute FSH suppression |
|
