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Sample GSM259917 Query DataSets for GSM259917
Status Public on Jan 29, 2008
Title FSH antibody for 4 days replicate 1
Sample type RNA
 
Source name Testis from Sprague Dawley male rat 161, Day 18 postnatal, FSH antibody for 4 days
Organism Rattus norvegicus
Characteristics male rat 161
Testis dissected from P18-day old Sprague Dawley rats
FSH antibody for 4 days
Treatment protocol Male day P18 rat were injected with anti-FSH for 4 days. Day P22 rat were euthanized and testis were removed according to IACUC-approved animal use protocols.
Extracted molecule total RNA
Extraction protocol About 20 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol chips were scanned using Agilent GeneArray Scanner G2500A
Description Chip ID=ingrid-161-U34A-010903. RNA from P18 day old rat testis.
Data processing Auto-scale ON (1-1000). Scaling = all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
 
Submission date Jan 28, 2008
Last update date Jan 28, 2008
Contact name Michael K Skinner
E-mail(s) skinner@mail.wsu.edu
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
 
Platform ID GPL85
Series (1)
GSE10293 Rat expression data after acute FSH suppression

Data table header descriptions
ID_REF Affymetrix Probe set ID
VALUE GCOS-calculated signal intensities
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE t-test p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A01157cds_s_at 12.9 A 0.5835963
A03913cds_s_at 15.8 A 0.9962338
A04674cds_s_at 9.7 A 0.42749014
A07543cds_s_at 45.8 A 0.50144374
A09811cds_s_at 376 P 0.8488925
A16585cds_s_at 5.7 A 0.44616836
A17753cds_s_at 2.7 A 0.39585376
A30543cds_s_at 11.1 A 0.76394403
A44407cds_at 3.2 A 0.4023636
AA108277_at 40.2 P 0.39158443
AA108308_i_at 0.7 A 0.37193665
AA108308_s_at 132.6 A 0.81504065
AA684537_at 97 A 0.72464186
AA684929_f_at 108.3 A 0.82826185
AA684960_f_at 65.8 A 0.74081594
AA684963_at 312.7 P 0.9354003
AA685112_at 287.7 P 0.9044029
AA685152_r_at 32.1 P 0.6117595
AA685376_f_at 14.5 A 0.864085
AA685876_at 89.9 A 0.44078395

Total number of rows: 8799

Table truncated, full table size 263 Kbytes.




Supplementary file Size Download File type/resource
GSM259917.CEL.gz 2.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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