|
Status |
Public on May 01, 2018 |
Title |
DMF biological rep1 |
Sample type |
RNA |
|
|
Source name |
Cells treated with DMF for 0h
|
Organism |
Mus musculus |
Characteristics |
cell type: Th17
|
Treatment protocol |
Cells were treated with 50 uM of DMF for 6 and 12 hours.
|
Growth protocol |
Cells were cultured in IMDM medium (10%FBS).
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy mini kit
|
Label |
biotin
|
Label protocol |
The protocol used for the samples run on the Clariom S mouse arrays was the Whole Transcript Plus assay from Affymetrix. The initial input of RNA was 100 ng
|
|
|
Hybridization protocol |
The WT Plus assay from Affymetrix fragments and labels 5.5 ug of ss-cDNA of which 2.3 ug is hybridized on the Clariom S mouse array with a constant temperature of 45° C for 16 hours while rotating at 60 rpm.
|
Scan protocol |
The samples were the stained and washed using the Affymetrix Fluidics 450 stations and recommended protocol. Then the Clariom S mouse arrays were then scanned by the Affymetrix GeneChip Scanner 3000 7G
|
Description |
JYW007
|
Data processing |
Data were background adjusted, normalized and summarized by the RMA method.
|
|
|
Submission date |
May 09, 2017 |
Last update date |
May 01, 2018 |
Contact name |
JUN YANG |
E-mail(s) |
jun.yang2@stjude.org
|
Organization name |
St Jude Children's Research Hospital
|
Department |
Surgery
|
Street address |
262 Danny Thomas Place
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE98733 |
Glutaminolysis fuels de novo glutathione synthesis, regulates redox homeostasis and directs T cell differentiation |
|