|
| Status |
Public on May 01, 2018 |
| Title |
DMF 12hour biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Cells treated with DMF for 12h
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Th17
|
| Treatment protocol |
Cells were treated with 50 uM of DMF for 6 and 12 hours.
|
| Growth protocol |
Cells were cultured in IMDM medium (10%FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kit
|
| Label |
biotin
|
| Label protocol |
The protocol used for the samples run on the Clariom S mouse arrays was the Whole Transcript Plus assay from Affymetrix. The initial input of RNA was 100 ng
|
| |
|
| Hybridization protocol |
The WT Plus assay from Affymetrix fragments and labels 5.5 ug of ss-cDNA of which 2.3 ug is hybridized on the Clariom S mouse array with a constant temperature of 45° C for 16 hours while rotating at 60 rpm.
|
| Scan protocol |
The samples were the stained and washed using the Affymetrix Fluidics 450 stations and recommended protocol. Then the Clariom S mouse arrays were then scanned by the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
JYW012
|
| Data processing |
Data were background adjusted, normalized and summarized by the RMA method.
|
| |
|
| Submission date |
May 09, 2017 |
| Last update date |
May 01, 2018 |
| Contact name |
JUN YANG |
| E-mail(s) |
jun.yang2@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Surgery
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE98733 |
Glutaminolysis fuels de novo glutathione synthesis, regulates redox homeostasis and directs T cell differentiation |
|
