|
| Status |
Public on Mar 31, 2008 |
| Title |
wildtype 3 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow macrophages
|
| Organism |
Mus musculus |
| Characteristics |
wildtype bone marrow macrophages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
(Qiagen, Venlo, the Netherlands). Contaminating genomic DNA was removed with the RNase-free DNase set (Qiagen, Venlo, the Netherlands) according the manufactures instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
|
| Scan protocol |
Scanning was performed according to the manufactures instructions as described in the eukaryotic section of the GeneChip Expression Analysis Technical Manual (Affymetrix, 2007)
|
| Description |
RIP140 knockout bone marrow macrophages
|
| Data processing |
Result files containing all relevant raw data were processed using the statistical Software SAS JMP7 Genomics3 (http://http://www.jmp.com/software/genomics/index.shtml). Raw fluorescence intensity values were normalized applying Mixed Model Normalization (Chu et al., 2002).
|
| |
|
| Submission date |
Feb 05, 2008 |
| Last update date |
Feb 07, 2008 |
| Contact name |
Inka Zschiedrich |
| Organization name |
German Cancer Research Center Heidelberg
|
| Department |
Molecular Metabolic Control
|
| Lab |
Molecular Metabolic Control
|
| Street address |
Im NeuenheimerFeld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6456 |
| Series (1) |
| GSE10386 |
Co-activator function of RIP140 for NFκB/p65-dependent cytokine gene |
|
