|
| Status |
Public on Sep 08, 2009 |
| Title |
Huh7_TGFbeta_minus_2 |
| Sample type |
RNA |
| |
|
| Source name |
hepatocellular carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
untreated with TGF-beta
|
| Treatment protocol |
Cells were treated with TGFbeta (R&D systems) and harvested after 48 hours of incubation.
|
| Growth protocol |
A human HCC cell line, Huh-7, was obtained from the Riken Bioresource Center (Tsukuba, Japan). The cell line was grown in Dulbecco’s modified Eagle medium (American Type Culture Collection) supplemented with 10% heat-inactivated fetal bovine serum at 37C in a 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from confluent 10cm culture dish using TRIzol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Refer to the Affymetrix Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the Affymetrix Assay System Manual
|
| Scan protocol |
Refer to the Affymetrix Assay System Manual
|
| Description |
Hepatocellular carcinoma cell lines treated or untreated with TGF-beta.
|
| Data processing |
Raw scan data were normalized using rma() function in Bioconductor's affy package (http://www.bioconductor.org/). For the analysis in the manuscript, the normalized signals were further collapsed into gene symbols by taking median values.
|
| |
|
| Submission date |
Feb 05, 2008 |
| Last update date |
Sep 08, 2009 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE10393 |
Integrative Transcriptome Analysis Reveals Common Molecular Subtypes of Human Hepatocellular Carcinoma (HT-HG_U133A) |
|
