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Status |
Public on Sep 25, 2019 |
Title |
soft palate muscle of patients with chronic tonsillitis 4 |
Sample type |
RNA |
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Source name |
soft palate muscle of patients with chronic tonsillitis
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Organism |
Homo sapiens |
Characteristics |
subject status: patients with chronic tonsillitis gender: male age: adult tissue: soft palate muscle molecule subtype: miRNA
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Treatment protocol |
Muscles were obtained from the excision soft palate in operation within 10min seperated from patients, immediately putted into liquid nitrogen.
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Growth protocol |
All patients with OSAHS and chronic tonsilitis were inpatients who had surgery in ENT depatment of the Second Affillited Hospital of Xi'an Jiaotong Univesity. 4 patients with OSAHS were received UPPP, and 4 patients with chronic tonsillitis received tonsillectomy.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
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Label |
Hy3
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Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
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Hybridization protocol |
The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual
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Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
Description |
liyuan
|
Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Four replicated spots of each miRNA probe were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs were identified through Fold Change filtering (>=2).
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Submission date |
May 24, 2017 |
Last update date |
Sep 25, 2019 |
Contact name |
Jin Hou |
E-mail(s) |
13319251982@126.com
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Phone |
+8613319251982
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Organization name |
The Second Affiliated Hospital of Xi'an Jiaotong University
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Department |
Otolaryngology- Head and Neck Department
|
Street address |
157 Xiwu Road
|
City |
Xi'an |
State/province |
Shaanxi |
ZIP/Postal code |
710004 |
Country |
China |
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Platform ID |
GPL19128 |
Series (1) |
GSE99239 |
Comparison of microRNA expression profiles in soft palate muscle of patients with OSAHS and chronic tonsillitis. |
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