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Sample GSM2637922 Query DataSets for GSM2637922
Status Public on Sep 25, 2019
Title soft palate muscle of patients with chronic tonsillitis 4
Sample type RNA
 
Source name soft palate muscle of patients with chronic tonsillitis
Organism Homo sapiens
Characteristics subject status: patients with chronic tonsillitis
gender: male
age: adult
tissue: soft palate muscle
molecule subtype: miRNA
Treatment protocol Muscles were obtained from the excision soft palate in operation within 10min seperated from patients, immediately putted into liquid nitrogen.
Growth protocol All patients with OSAHS and chronic tonsilitis were inpatients who had surgery in ENT depatment of the Second Affillited Hospital of Xi'an Jiaotong Univesity. 4 patients with OSAHS were received UPPP, and 4 patients with chronic tonsillitis received tonsillectomy.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
Label Hy3
Label protocol The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
 
Hybridization protocol The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.18.0) (Exiqon) according to array manual
Scan protocol The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Description liyuan
Data processing Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Four replicated spots of each miRNA probe were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs were identified through Fold Change filtering (>=2).
 
Submission date May 24, 2017
Last update date Sep 25, 2019
Contact name Jin Hou
E-mail(s) 13319251982@126.com
Phone +8613319251982
Organization name The Second Affiliated Hospital of Xi'an Jiaotong University
Department Otolaryngology- Head and Neck Department
Street address 157 Xiwu Road
City Xi'an
State/province Shaanxi
ZIP/Postal code 710004
Country China
 
Platform ID GPL19128
Series (1)
GSE99239 Comparison of microRNA expression profiles in soft palate muscle of patients with OSAHS and chronic tonsillitis.

Supplementary file Size Download File type/resource
GSM2637922_liyuan.gpr.gz 967.6 Kb (ftp)(http) GPR
Processed data are available on Series record

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