|
| Status |
Public on Mar 01, 2008 |
| Title |
MyoD-/-, 1999, 0hr, chip MG_U74Cv2 |
| Sample type |
other |
| |
|
| Source name |
Primary Myoblasts, Skeletal Muscle
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Balb/c MyoD-/-
Gender: Male
Karyotype: normal
|
| Growth protocol |
Growth medium: Ham's F10 Nutrient Mixture
Media supplements: 20% FBS, bFGF 250 ng/ml, 4% Penicillin (5x10E3 U/ml)-Streptomycin (5x10E3 ug/ml), 1% L-Glutamine 200 mM
Culture conditions: 37ºC, 5% CO2, 0.01% Collagen coated petri plate; Change with fresh media at day 3rd.
|
| Extracted molecule |
other |
| Extraction protocol |
Cell purification method: cultured
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.
|
| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E234
Stembase Experiment ID link: http://www.stembase.ca/?path=/browse/experiment&id=234
SCGP Sample ID: S343
SCGP Sample ID link: http://www.stembase.ca/?path=/browse/experiment&id=234#SAMPLE_352
Short description: Myogenic Primary Myoblast Time Course in vitro Differentiation into Myotubes for Ontario Genome Project 2004-05. , The Differentiation is leaded by removing the Proliferation Medium (Ham's F10 Medium + growth factors) and feeding with Differentiation Medium (DMEM hg + 2.5% Horse serum). , The expected data are: % Viability of cells; Total RNA extraction; BioAnalysis and MicroArray of Undifferentiated and Differentiated cells., Time point: 0 hr
Estimated purity: 100%
RNA concentration: 3325.8 ng/µL
Num cells for RNA prep: More than 2 x 10E+6
Sample volume: 27µL
|
| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpha2=0.06
|
| |
|
| Submission date |
Feb 07, 2008 |
| Last update date |
Feb 29, 2008 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL83 |
| Series (1) |
|
