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Status |
Public on May 31, 2017 |
Title |
Intestinal epithelial cells_VilCreER CtnnbloxEx3/+ KrasG12D/+, biological replicate 1 |
Sample type |
RNA |
|
|
Source name |
Epithelial cells, small intestine
|
Organism |
Mus musculus |
Characteristics |
genotype: VilCreER CtnnbloxEx3/+ KrasG12D/+
|
Treatment protocol |
To induce the expression of stabilized β-catenin and mutant Kras mice received 1 mg Tamoxifen on 5 consecutive days.
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Growth protocol |
Mice heterozygous for the CtnnbloxEx3 and KrasG12D allels were crossed to VilCreER or Xbp1sCreER mice. Mice were maintained on a mixed background.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by Qiagen RNeasy Mini Kit according to the manufacturer's protocol.
|
Label |
biotin
|
Label protocol |
GeneChip WT Terminal Labeling Kit.
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|
|
Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash and Stain Kit.
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Scan protocol |
Affimetrix GeneChip Scanner 3000 7G.
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Data processing |
Data were quantile normalized and log2 transformed using R/Bioconductor package limma
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|
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Submission date |
May 30, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Gabriela Salinas |
E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
Organization name |
Universitaetsmedizin Goettingen
|
Department |
Department of Human Genetics
|
Lab |
NGS Integrative Genomics
|
Street address |
Justus-von-Liebig-Weg 11
|
City |
Goettingen |
State/province |
Lower-Saxony |
ZIP/Postal code |
37077 |
Country |
Germany |
|
|
Platform ID |
GPL17777 |
Series (1) |
GSE99408 |
Gene expression profiles of intestinal tumors originating from different cell compartments |
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