|
| Status |
Public on May 31, 2017 |
| Title |
Intestinal epithelial cells_VilCreER CtnnbloxEx3/+ KrasG12D/+, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Epithelial cells, small intestine
|
| Organism |
Mus musculus |
| Characteristics |
genotype: VilCreER CtnnbloxEx3/+ KrasG12D/+
|
| Treatment protocol |
To induce the expression of stabilized β-catenin and mutant Kras mice received 1 mg Tamoxifen on 5 consecutive days.
|
| Growth protocol |
Mice heterozygous for the CtnnbloxEx3 and KrasG12D allels were crossed to VilCreER or Xbp1sCreER mice. Mice were maintained on a mixed background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Qiagen RNeasy Mini Kit according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
GeneChip WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash and Stain Kit.
|
| Scan protocol |
Affimetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Data were quantile normalized and log2 transformed using R/Bioconductor package limma
|
| |
|
| Submission date |
May 30, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Human Genetics
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Justus-von-Liebig-Weg 11
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37077 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17777 |
| Series (1) |
| GSE99408 |
Gene expression profiles of intestinal tumors originating from different cell compartments |
|
