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Status |
Public on Sep 06, 2017 |
Title |
MDAMB231_4175_sicontrol_2 |
Sample type |
RNA |
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Source name |
4175 cells, sicontrol
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 lung variant (4175)
|
Treatment protocol |
4175 cells were treated with sicontrol or sipgc1alpha as described here, materials and methods
|
Growth protocol |
4175 cells were cultured and grown as described in this paper, materials and methods
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from 4175 cells were isolated in tissue culture using an Aurum Total RNA Mini Kit.
|
Label |
Aurum Total RNA Mini Kit
|
Label protocol |
Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
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Hybridization protocol |
After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Human Clariom™ S (ThermoFisher)and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
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Scan protocol |
The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
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Description |
cell culture expression data from human lung metastatic 4175 cells treated with sicontrol
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Data processing |
Bioconductor. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls, *RPTR controls or probe sets named with 230*.
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Submission date |
Jun 01, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Julie St-Pierre |
E-mail(s) |
julie.st-pierre@mcgill.ca
|
Organization name |
McGill University
|
Department |
Department of Biochemistry
|
Street address |
3655 Promenade Sir William Osler
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3G 1Y6 |
Country |
Canada |
|
|
Platform ID |
GPL23159 |
Series (2) |
GSE99554 |
Expression data from a human lung metastatic cell line treated with siPGC1alpha or siControl |
GSE99557 |
Expression data from a lung metastatic cell line or metastatic explants in mouse and human |
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