|
| Status |
Public on Sep 06, 2017 |
| Title |
NMuMG-ErbB2/PGC-1alpha_Phenformin3 |
| Sample type |
RNA |
| |
|
| Source name |
lung metastasis, PGC1alpha, Phenformin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: metastasis lung tissue
cell line: NMuMG-ErbB2/PGC- 1alpha
|
| Treatment protocol |
lung were perfused with 50% optimal cutting temperature solution, diluted in PBS and removed and embedded in OCT at -80degC
|
| Growth protocol |
lung metastasis from mice treated with phenformin or PBS was extracted by laser capture microdissection (LCM) from slides
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lung metastasis RNA was isolated using LCM and pico RNA kit.
|
| Label |
pico RNA kit
|
| Label protocol |
Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
|
| |
|
| Hybridization protocol |
After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Mouse Clariom™ S (ThermoFisher) and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
|
| Scan protocol |
The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
|
| Description |
expression data of lung metastatic tissue from mice injected with PGC1alpha cells, treated with phenformin
|
| Data processing |
Bioconductor Oligo R package. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls or *RPTR controls.
|
| |
|
| Submission date |
Jun 01, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Julie St-Pierre |
| E-mail(s) |
julie.st-pierre@mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Department of Biochemistry
|
| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE99555 |
Expression data from lung metastasis |
| GSE99557 |
Expression data from a lung metastatic cell line or metastatic explants in mouse and human |
|
