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Sample GSM2645914 Query DataSets for GSM2645914
Status Public on Sep 06, 2017
Title NMuMG-ErbB2/VC_PBS_5
Sample type RNA
Source name lung metastasis, control, PBS
Organism Mus musculus
Characteristics tissue: metastasis lung tissue
cell line: NMuMG-ErbB2/VC (control)
Treatment protocol lung were perfused with 50% optimal cutting temperature solution, diluted in PBS and removed and embedded in OCT at -80degC
Growth protocol lung metastasis from mice treated with phenformin or PBS was extracted by laser capture microdissection (LCM) from slides
Extracted molecule total RNA
Extraction protocol Lung metastasis RNA was isolated using LCM and pico RNA kit.
Label pico RNA kit
Label protocol Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
Hybridization protocol After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Mouse Clariom™ S (ThermoFisher) and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
Scan protocol The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
Description expression data of lung metastatic tissue from mice injected with control cells, treated with PBS
Data processing Bioconductor Oligo R package. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls or *RPTR controls.
Submission date Jun 01, 2017
Last update date Jan 23, 2018
Contact name Julie St-Pierre
Organization name McGill University
Department Department of Biochemistry
Street address 3655 Promenade Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
Platform ID GPL23038
Series (2)
GSE99555 Expression data from lung metastasis
GSE99557 Expression data from a lung metastatic cell line or metastatic explants in mouse and human

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
ERCCmix1step1 5.372111576
ERCCmix1step10 4.647298459
ERCCmix1step11 7.102773715
ERCCmix1step12 3.626694008
ERCCmix1step13 3.864478536
ERCCmix1step14 4.833871428
ERCCmix1step15 4.30550253
ERCCmix1step16 3.487480399
ERCCmix1step17 4.501786552
ERCCmix1step18 4.189521047
ERCCmix1step19 5.516554889
ERCCmix1step2 6.914604125
ERCCmix1step20 4.076037521
ERCCmix1step21 6.320502812
ERCCmix1step22 7.586814175
ERCCmix1step3 5.289371405
ERCCmix1step4 4.777049105
ERCCmix1step5 5.00093077
ERCCmix1step6 3.549388105
ERCCmix1step7 4.930886547

Total number of rows: 28815

Table truncated, full table size 860 Kbytes.

Supplementary file Size Download File type/resource
GSM2645914_49_JPS100C10C10_01.CEL.gz 1.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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