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Sample GSM2645917 Query DataSets for GSM2645917
Status Public on Sep 06, 2017
Title LMTV_NMuMG-ErbB2/PGC-1alpha_ctrl_3
Sample type RNA
Source name LMTV, PGC1alpha, dh20
Organism Mus musculus
Characteristics cell line: LMTV_NMuMG-ErbB2/PGC- 1alpha
tissue: lung metastatis explants
Treatment protocol LMTV explants were treated with phenformin (100uM) or dh20 control for 4 days
Growth protocol LMTV explants were grown as described in this paper, materials and methods
Extracted molecule total RNA
Extraction protocol RNA from LMTV explants were isolated in tissue culture using an Aurum Total RNA Mini Kit.
Label Aurum Total RNA Mini Kit
Label protocol Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
Hybridization protocol After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Mouse Clariom™ S (ThermoFisher) and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
Scan protocol The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
Description cell culture
expression data of explants from LMTV injections of mice injected with PGC1alpha cells. explants treated with dh20 control for 4 days
Data processing Bioconductor. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls or *RPTR controls.
Submission date Jun 01, 2017
Last update date Jan 23, 2018
Contact name Julie St-Pierre
Organization name McGill University
Department Department of Biochemistry
Street address 3655 Promenade Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
Platform ID GPL23038
Series (2)
GSE99556 Expression data from lung metastatic explants
GSE99557 Expression data from a lung metastatic cell line or metastatic explants in mouse and human

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
ERCCmix1step1 5.184035499
ERCCmix1step10 4.811008995
ERCCmix1step11 6.042180927
ERCCmix1step12 4.134036574
ERCCmix1step13 4.841413269
ERCCmix1step14 5.655172115
ERCCmix1step15 4.001337267
ERCCmix1step16 3.611112267
ERCCmix1step17 4.223736648
ERCCmix1step18 4.443877766
ERCCmix1step19 5.972678625
ERCCmix1step2 5.808979414
ERCCmix1step20 4.261094087
ERCCmix1step21 6.373132529
ERCCmix1step22 7.876019437
ERCCmix1step3 5.698399217
ERCCmix1step4 5.354272878
ERCCmix1step5 5.565616987
ERCCmix1step6 2.97145599
ERCCmix1step7 4.784179323

Total number of rows: 28815

Table truncated, full table size 861 Kbytes.

Supplementary file Size Download File type/resource
GSM2645917_5.3_JPS045-3_01.CEL.gz 1.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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