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Sample GSM2645918 Query DataSets for GSM2645918
Status Public on Sep 06, 2017
Title LMTV_NMuMG-ErbB2/PGC-1alpha_phenformin_1
Sample type RNA
 
Source name LMTV, PGC1alpha, phenformin 4 days
Organism Mus musculus
Characteristics cell line: LMTV_NMuMG-ErbB2/PGC- 1alpha
tissue: lung metastatis explants
Treatment protocol LMTV explants were treated with phenformin (100uM) or dh20 control for 4 days
Growth protocol LMTV explants were grown as described in this paper, materials and methods
Extracted molecule total RNA
Extraction protocol RNA from LMTV explants were isolated in tissue culture using an Aurum Total RNA Mini Kit.
Label Aurum Total RNA Mini Kit
Label protocol Sense-strand cDNA was synthesized from 10 ng of total RNA and fragmentation and labeling were performed to produce ds DNA with the GeneChip® Pico Labeling Kit according to manufacturer’s instructions (Applied Biosystems)
 
Hybridization protocol After fragmentation and labeling, 4 µg DNA target was hybridized on GeneChip® Mouse Clariom™ S (ThermoFisher) and incubated at 450C in the Genechip® Hybridization oven 640 (ThermoFisher) for 17 hours at 60 rpm
Scan protocol The microarrays were finally scanned on a GeneChip® scanner 3000 (ThermoFisher).
Description cell culture
expression data of explants from LMTV injections of mice injected with PGC1alpha cells. explants treated with phenformin for 4 days
Data processing Bioconductor. The file 'normalized_data_allcontrols.txt' contains data for all probe sets including controls. The sample table does not include data for AFFX controls or *RPTR controls.
 
Submission date Jun 01, 2017
Last update date Jan 23, 2018
Contact name Julie St-Pierre
E-mail(s) julie.st-pierre@mcgill.ca
Organization name McGill University
Department Department of Biochemistry
Street address 3655 Promenade Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
 
Platform ID GPL23038
Series (2)
GSE99556 Expression data from lung metastatic explants
GSE99557 Expression data from a lung metastatic cell line or metastatic explants in mouse and human

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
ERCCmix1step1 5.107323722
ERCCmix1step10 4.737473132
ERCCmix1step11 5.818512689
ERCCmix1step12 4.081915166
ERCCmix1step13 4.740516477
ERCCmix1step14 5.518304264
ERCCmix1step15 4.111421861
ERCCmix1step16 3.449961919
ERCCmix1step17 4.263175494
ERCCmix1step18 4.491877881
ERCCmix1step19 5.970548862
ERCCmix1step2 5.628728396
ERCCmix1step20 4.150558863
ERCCmix1step21 6.755193634
ERCCmix1step22 8.133907664
ERCCmix1step3 5.464557217
ERCCmix1step4 4.889298083
ERCCmix1step5 5.044742936
ERCCmix1step6 2.714526831
ERCCmix1step7 4.82989312

Total number of rows: 28815

Table truncated, full table size 860 Kbytes.




Supplementary file Size Download File type/resource
GSM2645918_5.4_JPS046-3_01.CEL.gz 1.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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