|
Status |
Public on Jun 01, 2018 |
Title |
K562 cells transduced with pCDH-RUNX1-R135T |
Sample type |
RNA |
|
|
Source name |
K562 cells transduced with RUNX1-R135T
|
Organism |
Homo sapiens |
Characteristics |
cell line: K562 cell type: Chronic myelocytic leukemia cell line transduction: pCDH-RUNX1-R135T
|
Treatment protocol |
K562 cells were transduced with EV, RUNX1-WT and RUNX1-R135T plasmid. Cells were selected with 2 μg/ml puromycin for 2 weeks.
|
Growth protocol |
K562 cells were cultured in RPMI (GibCo, Invitrogen) and contained 10% FBS and 100 mg/mL streptomycin-penicillin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol total RNA isolation reagent (Invitrogen) by following manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix protocol.
|
Scan protocol |
Standard Affymetrix protocol.
|
Description |
RUNX1-R135T Experimental
|
Data processing |
Normalize with RMA by Affymetrix Expression Console Ver 1.2.
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|
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Submission date |
Jun 02, 2017 |
Last update date |
Jun 01, 2018 |
Contact name |
Yun-Shien Lee |
E-mail(s) |
bojack@mail.mcu.edu.tw
|
Organization name |
Ming Chuan Univ
|
Department |
Dept. Biotechnology
|
Lab |
GMRCL
|
Street address |
TauYau
|
City |
TauYau |
State/province |
Taiwan |
ZIP/Postal code |
115 |
Country |
Taiwan |
|
|
Platform ID |
GPL23126 |
Series (1) |
GSE99640 |
Functional study on the cooperation of ASXL1 and RUNX1 mutations for myeloid leukemia transformation |
|