NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2649112 Query DataSets for GSM2649112
Status Public on Jun 01, 2018
Title K562 cells transduced with pCDH-RUNX1-R135T
Sample type RNA
 
Source name K562 cells transduced with RUNX1-R135T
Organism Homo sapiens
Characteristics cell line: K562
cell type: Chronic myelocytic leukemia cell line
transduction: pCDH-RUNX1-R135T
Treatment protocol K562 cells were transduced with EV, RUNX1-WT and RUNX1-R135T plasmid. Cells were selected with 2 μg/ml puromycin for 2 weeks.
Growth protocol K562 cells were cultured in RPMI (GibCo, Invitrogen) and contained 10% FBS and 100 mg/mL streptomycin-penicillin.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Trizol total RNA isolation reagent (Invitrogen) by following manufacturer's instructions.
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description RUNX1-R135T
Experimental
Data processing Normalize with RMA by Affymetrix Expression Console Ver 1.2.
 
Submission date Jun 02, 2017
Last update date Jun 01, 2018
Contact name Yun-Shien Lee
E-mail(s) bojack@mail.mcu.edu.tw
Organization name Ming Chuan Univ
Department Dept. Biotechnology
Lab GMRCL
Street address TauYau
City TauYau
State/province Taiwan
ZIP/Postal code 115
Country Taiwan
 
Platform ID GPL23126
Series (1)
GSE99640 Functional study on the cooperation of ASXL1 and RUNX1 mutations for myeloid leukemia transformation

Data table header descriptions
ID_REF
VALUE Log2 RMA intensity

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 9.692325
AFFX-BkGr-GC04_st 8.392179
AFFX-BkGr-GC05_st 7.004443
AFFX-BkGr-GC06_st 5.73848
AFFX-BkGr-GC07_st 4.929063
AFFX-BkGr-GC08_st 4.231665
AFFX-BkGr-GC09_st 3.597039
AFFX-BkGr-GC10_st 3.397122
AFFX-BkGr-GC11_st 3.152214
AFFX-BkGr-GC12_st 2.731668
AFFX-BkGr-GC13_st 2.429274
AFFX-BkGr-GC14_st 2.286753
AFFX-BkGr-GC15_st 2.128467
AFFX-BkGr-GC16_st 2.204225
AFFX-BkGr-GC17_st 2.159599
AFFX-BkGr-GC18_st 2.284745
AFFX-BkGr-GC19_st 2.429274
AFFX-BkGr-GC20_st 2.581677
AFFX-BkGr-GC21_st 2.581677
AFFX-BkGr-GC22_st 3.152214

Total number of rows: 138745

Table truncated, full table size 3669 Kbytes.




Supplementary file Size Download File type/resource
GSM2649112_RUNX1-R135T.CEL.gz 22.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap