|
| Status |
Public on Feb 14, 2018 |
| Title |
GA-treated H1299,bio rep-1 |
| Sample type |
RNA |
| |
|
| Source name |
Lung cell line H1299, GA treated, 120 h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
cell line: H1299
tumor type: non-small cell lung carcinoma
treatment: GA treated, 120 h
|
| Treatment protocol |
Cells were treated with either 10 mM of GA or 5azaC for 120 h. Untreated cells of the same passage were used as controls.
|
| Growth protocol |
Cells were maintained in RPMI1640 medium supplemented with 10% FBS, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells were maintained at 37°C in a humidified 5% CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from cultured cells using RNeasy Mini-kit (Qiagen, MD, USA) followed the manufacturer’s protocol as described previously.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
H1299 cell line treated with GA for 120 h
|
| Data processing |
Data were processed using Affymetrix Expression Console Software (version 1.4) which normalizes array signals using Signal Space Transformation (SST) and robust multiarray averaging (RMA) algorithm.
probe group file: Clariom_S_Human.r1.pgf
meta-probeset file: Clariom_S_Human.r1.mps
|
| |
|
| Submission date |
Jun 13, 2017 |
| Last update date |
Feb 14, 2018 |
| Contact name |
Chia Huei Lee |
| E-mail(s) |
chlee124@nhri.edu.tw
|
| Organization name |
Taiwan National Health Research Institute
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd.
|
| City |
Zhunan Miaoli County |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE99993 |
Expression data from 5azaC- or gallic acid-treated human lung cancer H1299 cell line |
|
