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Status |
Public on Feb 14, 2018 |
Title |
GA-treated H1299, bio rep-2 |
Sample type |
RNA |
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Source name |
Lung cell line H1299, GA treated, 120 h
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Organism |
Homo sapiens |
Characteristics |
tissue: lung cell line: H1299 tumor type: non-small cell lung carcinoma treatment: GA treated, 120 h
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Treatment protocol |
Cells were treated with either 10 mM of GA or 5azaC for 120 h. Untreated cells of the same passage were used as controls.
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Growth protocol |
Cells were maintained in RPMI1640 medium supplemented with 10% FBS, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells were maintained at 37°C in a humidified 5% CO2 incubator
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA were extracted from cultured cells using RNeasy Mini-kit (Qiagen, MD, USA) followed the manufacturer’s protocol as described previously.
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Label |
biotin
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Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
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Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
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Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
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Description |
H1299 cell line treated with GA for 120 h
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Data processing |
Data were processed using Affymetrix Expression Console Software (version 1.4) which normalizes array signals using Signal Space Transformation (SST) and robust multiarray averaging (RMA) algorithm. probe group file: Clariom_S_Human.r1.pgf meta-probeset file: Clariom_S_Human.r1.mps
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Submission date |
Jun 13, 2017 |
Last update date |
Feb 14, 2018 |
Contact name |
Chia Huei Lee |
E-mail(s) |
chlee124@nhri.edu.tw
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Organization name |
Taiwan National Health Research Institute
|
Department |
National Institute of Cancer Research
|
Street address |
35, Keyan Rd.
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City |
Zhunan Miaoli County |
ZIP/Postal code |
114 |
Country |
Taiwan |
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Platform ID |
GPL23159 |
Series (1) |
GSE99993 |
Expression data from 5azaC- or gallic acid-treated human lung cancer H1299 cell line |
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