|
| Status |
Public on Jul 27, 2017 |
| Title |
PD 1 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: mononuclear cells
gender: male
age: 67 years old
diagnosis: Parkinson's disease
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from PBMC using an illustra RNAspin mini RNA isolation kit (GE Healthcare) according to the manufacturer's protocol. 100 ng of total RNA was carried out to First-strand cDNA synthesis using GeneChip® WT PLUS Reagent Kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Fragmented single-strand cDNA were subjected to 3' terminal labeling with biotin using GeneChip® WT PLUS Reagent Kit in accordance with manufacturer's protocol.
|
| |
|
| Hybridization protocol |
5.5 μg of fragmented and biotin-labeled single-strand cDNA were hybridized on ClariomTM D array (human) for 16 h at 45°C with rotation at 60 rpm using GeneChip® Hybridization Oven 645.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G (Affymetrix).
|
| Data processing |
The data were analyzed with Expression Console version 1.4.1 (Affymetrix) using default settings.
|
| |
|
| Submission date |
Jun 14, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Shuji Shimoyama |
| E-mail(s) |
shuji-s@hirosaki-u.ac.jp
|
| Phone |
+81-172-39-5138
|
| Organization name |
Hirosaki University Graduate School of Medicine
|
| Department |
Department of Neurophysiology
|
| Street address |
5 Zaifu-cho
|
| City |
Hirosaki |
| State/province |
Aomori |
| ZIP/Postal code |
036-8562 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE100054 |
Expression profiling of peripheral blood mononuclear cells in normal controls and patients with Parkinson's disease |
|
